class: center, middle, inverse, title-slide .title[ # Larger prokaryotes grow faster only at high temperatures ] .subtitle[ ## Dylan Padilla | ✉️
dpadil10@asu.edu
🔗
https://dylanpadilla.netlify.app/
Evolution 2024
] --- <style type="text/css"> pre { max-height: 200px; overflow-y: visible; overflow-x: visible; scrollbar-color: #007 #bada55; } pre[class] { max-height: 100px; } .scroll-100 { max-height: 100px; overflow-y: visible; } </style> # Acknowledgment .pull-left[ <center> <img src = "imgs/Kerry.png" width=400 heigth=400 /><br> Dr Kerry Geiler-Samerotte </center> ] .pull-right[ <center> <img src = "imgs/Lynch.jpeg" width=350 heigth=350 /><br> Dr Michael Lynch </center> ] --- # Outline <br/> ## Relationship between growth and size - Theoretical predictions - Empirical support -- ## Methods - Data source - Description of variables -- ## Current evidence - Striking observations - Analysis of findings --- class: center, middle background-image: url("imgs/bg-img.png") # Evolution of growth and size --- # Relationship between growth and size ## Metabolism governs the rate at which organisms transform energy into biological work and growth --- # Relationship between growth and size ## Metabolism governs the rate at which organisms transform energy into biological work and growth .pull-left[ <center> <img src = "imgs/elephant.png" width=600 height=480/> </center> ] -- .pull-right[ <br><br> <center> <img src = "imgs/mouse.png" width=200 height=200/> </center> ] --- # Prokaryotes defy theoretical predictions <br> .pull-left[ <center> <img src = "imgs/lynch.png" width = 650 height = 370 /> </center> ] .pull-right[ <center> <img src = "imgs/lynch2.png" width = 650 height = 400 /> </center> ] [Lynch et al., (2022)](https://www.nature.com/articles/s41598-022-23626-7) --- # Prokaryotes defy theoretical predictions <br> .pull-left[ <center> <img src = "imgs/marshall_2022.png" width = 500 height = 400 /><br> </center> [Marshall et al., (2022)](https://www.pnas.org/doi/full/10.1073/pnas.2200713119) ] -- .pull-right[ <center> <img src='https://media1.tenor.com/m/t5xv1FrpIc4AAAAd/pawpaw-thinking.gif' width=400 height=400/> </center> ] --- # Methods .pull-left[ <center> <img src = "imgs/repository.png"/><br> <img src = "imgs/literature1.png"/> </center> ] .pull-right[ <center> <img src = "imgs/literature2.png"/><br> <img src = "imgs/literature3.png"/> </center> ] --- # Methods .pull-left[ <br> <ul> <li> Minimum doubling time </li>$$(log_{10} h)$$ <li> Cell volume </li>$$(log_{10} \mu m^3)$$ <li> Cell diameter </li>$$(log_{10} \mu m)$$ </ul> ] .pull-right[ <center> <img src="index_files/figure-html/unnamed-chunk-3-1.png" width="90%" style="display: block; margin: auto;" /> </center> ] --- class: center, middle background-image: url("imgs/cells.png") background-size: cover # Results # Growth v Cell Size --- .pull-left[ <img src="index_files/figure-html/unnamed-chunk-5-1.png" style="display: block; margin: auto;" /> ] .pull-right[ <img src="index_files/figure-html/unnamed-chunk-8-1.png" style="display: block; margin: auto;" /> ] --- .pull-left[ <img src="index_files/figure-html/unnamed-chunk-9-1.png" style="display: block; margin: auto;" /> ] .pull-right[ <img src="index_files/figure-html/unnamed-chunk-10-1.png" style="display: block; margin: auto;" /> ] --- # Methods .pull-left[ ## Metabolism of protein synthesis ## "Translation Machinery" <br> <ul> <li> Number of ribosomal RNA genes (rRNA) </li><br> <li> Number of transfer RNA genes (tRNA) </li> </ul> ] .pull-right[ <center> <img src='imgs/translation.png' width=490 height=500/> </center> ] --- class: center, middle background-image: url("imgs/ribosomes.jpeg") background-size: cover # Results # Translation machinery --- <img src="index_files/figure-html/unnamed-chunk-11-1.png" width="60%" style="display: block; margin: auto;" /> --- <img src="index_files/figure-html/unnamed-chunk-12-1.png" width="60%" style="display: block; margin: auto;" /> --- class: center, middle background-image: url("imgs/temp.jpeg") background-size: cover <br><br> <center> <font size='10'>How does temperature scale with size and growth?</font> <center> --- # Methods <img src="index_files/figure-html/unnamed-chunk-13-1.png" width="50%" style="display: block; margin: auto;" /> --- # Methods <img src="index_files/figure-html/unnamed-chunk-14-1.png" width="50%" style="display: block; margin: auto;" /> --- # Methods <img src="index_files/figure-html/unnamed-chunk-15-1.png" width="50%" style="display: block; margin: auto;" /> --- # Methods <img src="index_files/figure-html/unnamed-chunk-16-1.png" width="50%" style="display: block; margin: auto;" /> --- class: center, middle background-image: url("imgs/temp.jpeg") background-size: cover <br><br> <center> <font size='10'>Results</font> <font size='10'>Temperature v Growth and Cell Size</font> <center> --- <br> <img src="index_files/figure-html/unnamed-chunk-18-1.png" style="display: block; margin: auto;" /> --- <br> <img src="index_files/figure-html/unnamed-chunk-19-1.png" style="display: block; margin: auto;" /> --- <br> <img src="index_files/figure-html/unnamed-chunk-20-1.png" style="display: block; margin: auto;" /> --- <br> <img src="index_files/figure-html/unnamed-chunk-21-1.png" style="display: block; margin: auto;" /> --- <br> <img src="index_files/figure-html/unnamed-chunk-22-1.png" style="display: block; margin: auto;" /> --- <br> <img src="index_files/figure-html/unnamed-chunk-23-1.png" style="display: block; margin: auto;" /> --- <br> <img src="index_files/figure-html/unnamed-chunk-25-1.png" width="80%" style="display: block; margin: auto;" /> --- class: center, middle background-color: black # Questions? ## These slides were made with the xaringan 📦 of <svg viewBox="0 0 581 512" style="position:relative;display:inline-block;top:.1em;fill:skyblue;height:1.3em;" xmlns="http://www.w3.org/2000/svg"> <path d="M581 226.6C581 119.1 450.9 32 290.5 32S0 119.1 0 226.6C0 322.4 103.3 402 239.4 418.1V480h99.1v-61.5c24.3-2.7 47.6-7.4 69.4-13.9L448 480h112l-67.4-113.7c54.5-35.4 88.4-84.9 88.4-139.7zm-466.8 14.5c0-73.5 98.9-133 220.8-133s211.9 40.7 211.9 133c0 50.1-26.5 85-70.3 106.4-2.4-1.6-4.7-2.9-6.4-3.7-10.2-5.2-27.8-10.5-27.8-10.5s86.6-6.4 86.6-92.7-90.6-87.9-90.6-87.9h-199V361c-74.1-21.5-125.2-67.1-125.2-119.9zm225.1 38.3v-55.6c57.8 0 87.8-6.8 87.8 27.3 0 36.5-38.2 28.3-87.8 28.3zm-.9 72.5H365c10.8 0 18.9 11.7 24 19.2-16.1 1.9-33 2.8-50.6 2.9v-22.1z"></path></svg>. To get the code, visit https://github.com/Dylan-Padilla/EVO2024/blob/main/index.Rmd